A simple, precise, accurate and reproducible uv-spectrophotometric method has been developed for the simultaneous estimation of bromhexine hydrochloride (BH) and phenylephrine hydrochloride (PL) in their combined pharmaceutical dosage form. This method utilizes methanol as a solvent and λmax of bromhexine hydrochloride and phenylephrine hydrochloride selected for analysis was found to be 250 nm and 275 nm respectively. Linearity was observed in the concentration range of 5-30 μg/ml for bromhexine HCl (r2 = 0.998) and 10-60 μg/ml for phenylephrine HCl (r2 = 0.999). The method was validated by statistically and by recovery study. The mean % recovery was 98.5-101.66% and 98.87-101.46% for bromhexine HCl and phenylephrine HCl respectively. Developed method was applied to marketed formulation. The results were found to be within acceptance criteria according to ICH guideline.
Loading....